This textbook has been designed to meet the needs of B.Sc. Fourth Semester students of Zoology as per the Common Minimum Syllabus prescribed for all Uttar Pradesh State Universities and Colleges under the recommended National Education Policy 2020 (NEP 2020). It comprehensively covers two papers, namely theory paper on Gene Technology, Immunology and Computational Biology and practical paper on Genetic Engineering and Counselling Lab. While this textbook gives a thorough overview of Gene Technology, Immunology and Computational Biology, it aptly covers important topics such as principles of gene manipulation, application of genetic engineering and immune system